Amino acid substitutions in the neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens
Identifieur interne : 000481 ( Main/Exploration ); précédent : 000480; suivant : 000482Amino acid substitutions in the neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens
Auteurs : Jing Lv ; Liangmeng Wei ; Yan Yang ; Bingxiao Wang ; Wei Liang ; Yuwei Gao ; Xianzhu Xia ; Lili Gao ; Yumei Cai ; Peiqiang Hou ; Huili Yang ; Airong Wang ; Rong Huang ; Jing Gao ; Tongjie ChaiSource :
- Veterinary Research [ 0928-4249 ] ; 2015-12.
Abstract
Cases of H9N2 avian influenza virus (AIV) in poultry are increasing throughout many Eurasian countries, and co-infections with other pathogens have resulted in high morbidity and mortality in poultry. Few studies have investigated the genetic factors of virus airborne transmission which determine the scope of this epidemic. In this study, we used specific-pathogen-free chickens housed in isolators to investigate the airborne transmissibility of five recombinant H9N2 AIV rescued by reverse genetic technology. The results show that airborne transmission of A/Chicken/Shandong/01/2008 (SD01) virus was related to the neuraminidase (NA) gene, and four amino acid mutations (D368E, S370L, E313K and G381D) within the head region of the SD01 NA, reduced virus replication in the respiratory tract of chickens, reduced virus NA activity, and resulted in a loss of airborne transmission ability in chickens. Similarly, reverse mutations of these four amino acids in the NA protein of r01/NASS virus, conferred an airborne transmission ability to the recombinant virus. We conclude that these four NA residues may be significant genetic markers for evaluating potential disease outbreak of H9N2 AIV, and propose that immediate attention should be paid to the airborne transmission of this virus.
Url:
DOI: 10.1186/s13567-014-0142-3
Affiliations:
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neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens</title><author><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name></author><author><name sortKey="Wei, Liangmeng" sort="Wei, Liangmeng" uniqKey="Wei L" first="Liangmeng" last="Wei">Liangmeng Wei</name></author><author><name sortKey="Yang, Yan" sort="Yang, Yan" uniqKey="Yang Y" first="Yan" last="Yang">Yan Yang</name></author><author><name sortKey="Wang, Bingxiao" sort="Wang, Bingxiao" uniqKey="Wang B" first="Bingxiao" last="Wang">Bingxiao Wang</name></author><author><name sortKey="Liang, Wei" sort="Liang, Wei" uniqKey="Liang W" first="Wei" last="Liang">Wei Liang</name></author><author><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name></author><author><name sortKey="Gao, Lili" sort="Gao, Lili" uniqKey="Gao L" first="Lili" last="Gao">Lili Gao</name></author><author><name sortKey="Cai, Yumei" sort="Cai, Yumei" uniqKey="Cai Y" first="Yumei" last="Cai">Yumei Cai</name></author><author><name sortKey="Hou, Peiqiang" sort="Hou, Peiqiang" uniqKey="Hou P" first="Peiqiang" last="Hou">Peiqiang Hou</name></author><author><name sortKey="Yang, Huili" sort="Yang, Huili" uniqKey="Yang H" first="Huili" last="Yang">Huili Yang</name></author><author><name sortKey="Wang, Airong" sort="Wang, Airong" uniqKey="Wang A" first="Airong" last="Wang">Airong Wang</name></author><author><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name></author><author><name sortKey="Gao, Jing" sort="Gao, Jing" uniqKey="Gao J" first="Jing" last="Gao">Jing Gao</name></author><author><name sortKey="Chai, Tongjie" sort="Chai, Tongjie" uniqKey="Chai T" first="Tongjie" last="Chai">Tongjie Chai</name></author></analytic><idno type="DOI">10.1186/s13567-014-0142-3</idno><series><title level="j">Veterinary Research</title><idno type="ISSN">0928-4249</idno><imprint><date type="datePub">2015-12</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Cases of H9N2 avian influenza virus (AIV) in poultry are increasing throughout many Eurasian countries, and co-infections with other pathogens have resulted in high morbidity and mortality in poultry. Few studies have investigated the genetic factors of virus airborne transmission which determine the scope of this epidemic. In this study, we used specific-pathogen-free chickens housed in isolators to investigate the airborne transmissibility of five recombinant H9N2 AIV rescued by reverse genetic technology. The results show that airborne transmission of A/Chicken/Shandong/01/2008 (SD01) virus was related to the neuraminidase (NA) gene, and four amino acid mutations (D368E, S370L, E313K and G381D) within the head region of the SD01 NA, reduced virus replication in the respiratory tract of chickens, reduced virus NA activity, and resulted in a loss of airborne transmission ability in chickens. Similarly, reverse mutations of these four amino acids in the NA protein of r01/NASS virus, conferred an airborne transmission ability to the recombinant virus. We conclude that these four NA residues may be significant genetic markers for evaluating potential disease outbreak of H9N2 AIV, and propose that immediate attention should be paid to the airborne transmission of this virus.</p> </div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Cai, Yumei" sort="Cai, Yumei" uniqKey="Cai Y" first="Yumei" last="Cai">Yumei Cai</name><name sortKey="Chai, Tongjie" sort="Chai, Tongjie" uniqKey="Chai T" first="Tongjie" last="Chai">Tongjie Chai</name><name sortKey="Gao, Jing" sort="Gao, Jing" uniqKey="Gao J" first="Jing" last="Gao">Jing Gao</name><name sortKey="Gao, Lili" sort="Gao, Lili" uniqKey="Gao L" first="Lili" last="Gao">Lili Gao</name><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><name sortKey="Hou, Peiqiang" sort="Hou, Peiqiang" uniqKey="Hou P" first="Peiqiang" last="Hou">Peiqiang Hou</name><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><name sortKey="Liang, Wei" sort="Liang, Wei" uniqKey="Liang W" first="Wei" last="Liang">Wei Liang</name><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name><name sortKey="Wang, Airong" sort="Wang, Airong" uniqKey="Wang A" first="Airong" last="Wang">Airong Wang</name><name sortKey="Wang, Bingxiao" sort="Wang, Bingxiao" uniqKey="Wang B" first="Bingxiao" last="Wang">Bingxiao Wang</name><name sortKey="Wei, Liangmeng" sort="Wei, Liangmeng" uniqKey="Wei L" first="Liangmeng" last="Wei">Liangmeng Wei</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Yang, Huili" sort="Yang, Huili" uniqKey="Yang H" first="Huili" last="Yang">Huili Yang</name><name sortKey="Yang, Yan" sort="Yang, Yan" uniqKey="Yang Y" first="Yan" last="Yang">Yan Yang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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